Emerging Single-cell Approaches to Understand HIV in the Central Nervous System.

TitleEmerging Single-cell Approaches to Understand HIV in the Central Nervous System.
Publication TypeJournal Article
Year of Publication2022
AuthorsCorley MJ, Farhadian SF
JournalCurr HIV/AIDS Rep
Volume19
Issue1
Pagination113-120
Date Published2022 Feb
ISSN1548-3576
Abstract

PURPOSE OF REVIEW: This review highlights emerging single-cell sequencing methods relevant to translational studies of HIV in the central nervous system (CNS), summarizes limited single-cell studies of HIV in the CNS, and discusses opportunities for future HIV translational CNS studies.

RECENT FINDINGS: Innovative methods utilizing single-cell technologies have advanced the study of genomes, proteomes, transcriptomes, and epigenomes at an enhanced resolution and depth. Single-cell analyses of central nervous system tissue, including autopsy brain and CSF cells, may shed light on CNS perturbations in people living with HIV. New strategies can distinguish distinct molecular identifies of rare infected cells at single-cell level, suggesting an opportunity to uncloak the molecular identity of hidden HIV in the CNS reservoir. Adoption of multimodal "omics" analyses to translational HIV studies and tissue compartments beyond blood will be critical to advancing our understanding of viral establishment, persistence, and eradication.

DOI10.1007/s11904-021-00586-7
Alternate JournalCurr HIV/AIDS Rep
PubMed ID34822063
PubMed Central IDPMC8613726
Grant ListUL1 TR001863 / TR / NCATS NIH HHS / United States